Biotransformation of Raw Mango Seed Waste into Bacterial Hydrolytic Enzymes Enhancement Via Solid State Fermentation Strategy.

Abstract

Solid waste generation is a huge contributor to environmental pollution issues, and food wastes are prominent in this category due to their large generation on a day-to-day basis. Thus, the settlement of daily food waste is one of the major constraints and needs innovative manufacturingsheme to valorize solid waste insustainable manner. Moreover,these food wastes are rich in organic content, which has promising scope for their value-added products. In the present study, raw mango seed waste has been biotransformed to produce bacterial hydrolytic enzymes as feedstock. On investigating the impact of substrate, the highest bacterial cellulase production was recorded to be 18IU/gds FP (filter paper) in 24h of microbial incubation at 5g of substrate in solid-state fermentation (SSF). Furthermore, at 40°C and pH 6.0, 23IU/gds FP enzyme could be produced in 24h of SSF. Beside this, on comparing the influence of inorganic and organic nitrogen sources, urea has been found to provide better cellulase production, which yielded 28IU/gds FP in 24h of incubation, along with 77IU/gds BG (β-glucosidase) and 89IU/gds EG (endoglucanase). On the other hand, Tween-40 and Tween-80, two different surfactants, were employed at a 1.0% concentration for 24h of incubation. It was noticed that Tween-80 showed complete enzyme activity at 24h, which was found to be relatively superior to that of Tween-40. This study may have potential utility in enzyme production using mango seed as a food waste for various industrial applications.