Abstract
Biotransformation of progesterone using Absidia griseolla var. igachii and Rhizomucor pusillus strains as biocatalysts were investigated. Microbial hydroxylation of progesterone by A. griseolla produced two hydroxylated pregnane-like steroids. The metabolites identified as 6β,14α-dihydroxyprogesterone and 7α,14α-dihydroxyprogesterone. R. pusillus produced 6β,11α-dihydroxyprogesterone with excellent yield (65.5%) and 7α,14α-dihydroxyprogesterone. These metabolites were purified by TLC followed by their identification through 1H, 13C NMR and other spectroscopic data.
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