Biotransformation of aflatoxin B1 by a novel strain Brevundimonas sp. LF-1

Abstract

Aflatoxin B1 (AFB1) is a potent mycotoxin and a common source of food and feed contamination, posing risks to both human and animal health. The biotransformation of AFB1 has been proven to be a promising approach to control AFB1 contamination. In this study, Brevundimonas sp. LF-1 capable of degrading AFB1 was isolated from a corn-planted soil sample. Strain LF-1 could degrade 86.90% of 2 mg L−1 AFB1 after incubation in Luria-Bertani (LB) medium at 30 °C for 72 h and exhibited high performance when exposed to up to 10.0 mg L−1 AFB1. The optimum pH and temperature were 7.0–10.0 and 30 °C, respectively. The results of the in vitro cytotoxicity assay showed that the degradation products had considerably (p < 0.05) less harmful effects than the parent AFB1. Additionally, strain LF-1 possessed the bioremediation potential of AFB1 contamination for biocontrol strategies in animal fodder. Two putative novel AFB1-degrading enzymes, peroxiredoxin 1 and peroxiredoxin 2, were identified in the genome of strain LF-1. Comparative genomics indicated that the peroxiredoxin enzyme was widely distributed in the genus Brevundimonas. The comprehensive examination of strain LF-1 has outstanding potential for the development of detoxifying agents for AFB1 in the food and feed industries.