Abstract
A continuous discovery of novel practices for in vitro plant culture is essential to promote better plant growth. An alternative approach to the conventional micropropagation practice could be achieved through biotization by supplying selected diazotrophs to the plant materials. Due to this considerable potential, an artificial symbiosis was created between the Herbaspirillum seropedicae strain Z78 (ATCC 35893) and in vitro oil palm (Elaeis guineensis Jacq.). This experiment was carried out with the aim of studying the effects of H. seropedicae inoculation on growth of oil palm calli and embryogenic calli. A total of 4 treatments were tested for calli and embryogenic calli of oil palm on modified Murashige–Skoog medium, namely: T1: 100 % nitrogen source + 100 % 2,4D; T2: 25 % nitrogen source + 10 % 2,4D; T3: Z78 sonicated cell + 25 % nitrogen source + 10 % 2,4D and T4: Z78 pellet cell + 25 % nitrogen source + 10 % 2,4D. The ability of the inoculum to produce phytohormone indole-3-acetic acid was detected by ultra-performance liquid chromatography. Scanning electron microscopy and transmission electron microscopy showed successful artificial symbiosis of calli and embryogenic calli-bacterium. This study has demonstrated that Z78 pellet cell and sonicated cell have the potential to promote growth of in vitro calli and embryogenic calli of oil palm under symbiosis conditions.
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