Abstract
The quantitative detection of biomarkers in protein‐rich samples using fluorescent probes is usually hampered by nonspecific fluorescence as a result of nonspecific probe–protein interactions. In this paper, we report a biotinylated fluorescent probe that is encapsulated in avidin protein, which can generate very specific fluorescence in blood serum by blocking out nonspecific dye–protein interactions. This approach was applied successfully to quantify glucose concentrations in blood serum.
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