Abstract
Bacillus sphaericus produced larger amounts of biotin when its precursor was added to the medium after 24-hr cultivation than when it was added before cultivation. Actithiazic acid (ACM)-and/or 5-(2-thienyl)-valeric acid (TVA)-resistant mutants were induced from B. sphaericus by treatment with N-methyl-N′-nitro-N-nitrosoguanidine. ACM- and TVA-resistant mutant, AB 12, excreted 7.1 times more biotin than the wild type strain. The TVA-resistant mutant, A 16, excreted 11.9 times more total biotin than the wild type strain. The reaction conditions for biotin production using resting cells of strain AB 12 were investigated. After 48-hr reaction, strain AB 12 accumulated 9.5 μg/ml of biotin under the optimum conditions.
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