Biotin-functionalized silica nanoparticles loaded with Erythrosine B asselective photodynamic treatment for Glioblastoma Multiforme

Abstract

In this contribution, we report the development of a biotin-functionalized silica nanoparticle loaded with Erythrosine B (ERY) to act as a selective cancer nanoplatform via Photodynamic Therapy. Amino silica nanoparticles were synthesized by the co-condensation method and further functionalized via amide linkage with biotin. The silica nanoparticles (mean diameter ∼120 nm) were loaded with ERY yieldings of 34.9 mg g−1 and 16.7 mg g−1, respectively, for the non-biotinylated (ERY/SiNP) and biotinylated (ERY/SiNP-B). The adsorption followed two consecutive first-order reactions, and the isotherms fitted to the Langmuir (SiNP) and Freundlich (SiNP-B) models. The ERY is strongly attached to the silica nanoparticle, which prevents their premature leakage to the biological fluids. The well-established effect of the uric acid as a singlet oxygen scavenger was used to show the 1O2 formation by the nanoplatforms. Trypan blue and MTT cytotoxic assays have shown that ERY/SiNP and ERY/SiNP-B samples are potential systems for PDT applications against Gliobastoma Multiforme (GBM) cancer cells vanishing around 50 % of T98G cells.