Abstract

The minimal biotin requirement for both maximum growth and normal morphological development of Arthrobacter globiformis 425 was found to be about 0.3 ng/ml medium. Morphological changes associated with biotin nutrition were investigated by light and electron microscopy. The aberrant form induced by biotin deficiency consisted of several membrane-bound bodies embedded in a thick matrix which appeared distinct from the wall of the normal organism. Both cell types resisted lysis by tonicity changes but the aberrant form showed greater resistance than the normal cell to destruction by sonication.

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