Abstract
BackgroundThe epidermal mucus of fish contains various bioactive compounds, which play a crucial role in the animal defense mechanisms. However, the mucus from giant mudskipper is not well characterized for its biochemical and pharmacological effects. This study for the first time analyzed the mucus extracts of this species for antimicrobial (against human pathogen) and hemolytic activities. Fourier-transform infrared spectrum analysis (FT-IR) and SDS-PAGE of mucus extracts were also carried out.ResultsThe protein content in Periophthalmodon schlosseri (Gobiidae: Oxudercine) mucus extracts was 0.66 ± 0.04 mg/ml. The mucus extracts of P. schlosseri showed antibacterial activity against all eight human pathogenic bacteria and four fungal strains tested in this study. The hemolytic activities against chicken and human blood cells were observed with maximum activity for dichloromethane extracts. The FT-IR spectrum of mucus extract of P. schlosseri showed the presence of functional groups, evident protein bands of various molecular weights ranging from 14 kDa to 97 kDa.ConclusionThe obtained results suggested that the mucus of this species has proteinaceous compounds responsible for antimicrobial and hemolytic activities.
Highlights
The epidermal mucus of fish contains various bioactive compounds, which play a crucial role in the animal defense mechanisms
Sample collection and preparation of mucus extract Specimens of the giant mudskipper Periophthalmodon schlosseri were freshly collected from Pichavaram, southeast coast of India
The mucus extract A showed the highest inhibition zone against Ps. aeruginosa (13 mm), whereas the lowest was observed against S. aureus (8 mm)
Summary
The epidermal mucus of fish contains various bioactive compounds, which play a crucial role in the animal defense mechanisms. The mucus from giant mudskipper is not well characterized for its biochemical and pharmacological effects. This study for the first time analyzed the mucus extracts of this species for antimicrobial (against human pathogen) and hemolytic activities. Fourier-transform infrared spectrum analysis (FT-IR) and SDS-PAGE of mucus extracts were carried out. Fish lives in an environment which is a source of microorganisms that can be potentially pathogenic. Skin or mucus of fish contains biologically active compounds with a potential against human pathogens. The mucus is composed of chemical substances secreted from the epithelial cells covering the epidermal surface (Rakers et al, 2013). Mucus contains mucin-like substances which have an antibiotic potential (Ingram, 1980) that reduces the
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