Biotic and abiotic factors regulating forest floor CO 2 flux across a range of forest age classes in the southern Appalachians

Abstract

Summary We measured forest floor CO 2 flux in three age classes of forest in the southern Appalachians: 20-year-old, 85-year-old, and old-growth. Our objectives were to quantify differences in forest floor CO 2 flux among age classes, and determine the relative importance of abiotic and biotic driving variables. Forest floor CO 2 flux was measured using an openflow infrared gas analyzer measurement system for 24 h periods and samples were taken every 2 months over a 2-year period. Litter/soil interface, soil temperature (5 cm depth), soil moisture (%), forest floor moisture (%), forest floor mass, fine root (⩽2 mm) mass, coarse root mass (>2 mm), forest floor C and N (%), fine root C and N, coarse root C and N, and soil N and C were co-measured during each sample period. Results showed significant nonlinear relationships ( r 2 =0.68 to 0.81) between litter/soil interface temperature and forest floor CO 2 flux for all three forest age classes, but no differences in temperature response parameters. These results indicated no differences in forest floor CO 2 flux among age classes. Considerable temporal variation in abiotic and biotic variables was observed within and among forests. Biotic variables correlated with forest floor CO 2 flux included indices of litter and root quality. Differences in biotic variables correlated with forest floor CO 2 flux among forests may have been related to shifts in the relative importance of heterotrophic and autotrophic respiration components to overall forest floor CO 2 flux.