Abstract
Plantlets of loblolly pine were regenerated and converted to soil by somatic polyembryogenesis (SPE). SPE was evoked in cells from an embryonal–suspensor mass whose nuclei were color–coded by a double–staining method. Color–coding with cell suspension cultures distinguished a free–nuclear stage, initial physiological states and the ultimate fate of each cell for estimates of yield based on the initial properties of embryogenic and nonembryogenic cell masses. Somatic embryos were encapsulated and stored in an alginate gel or in liquid nitrogen.
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