Biotechnology for remediation and restoring the ecological status of soil in the places of permanent disposition of the troops by oil-destructor preparation on the basis of the microbial-plant association

Abstract

The object of the study is a preparation of ecotoxicant destructor based on bacterial strains of Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas délhiensis and associations of nodule bacteria Rhizobium loti with Lotus corniculatus. It is established, that during the simultaneous use of the bacteria and the legume Lotus corniculatus the rate of oxidation of petroleum hydrocarbons increases three times in comparison with the degradation rate of the pollutant after the separate use of each bacterial strain. We have received the preparation of ecotoxicant destructor – dry heterogeneous mass consisting of viable microbial cells of strains of P. delhiensis and R. lotus, seeds of leguminous plant Lotus corniculatus and delignified sawdust. Oil destructive activity of the preparation is proved in the course of microfield experiments. The degradation of ecotoxicants is proved by capillary gas chromatography-mass spectrometry with mass-selective detection. Bacteria of the strains, included in the preparation, are non-pathogenic for humans and animals, biocompatible, environmentally safe, stable, unpretentious to nutritional needs, technological, do not persist in environmental objects in the absence of a substrate for destruction. The technology of the production of the preparation is developed. The results of these studies demonstrate the possibility of practical use of the degradative potential of the preparation in the course of the implementation of the measures for soil reclamation, cleaning up ecotoxicants and restoring its ecological status.