Biotechnology Approaches on Characterization, Mass Propagation, and Breeding of Indonesian Orchids Dendrobium lineale (Rolfe.) and Vanda tricolor (Lindl.) with Its Phytochemistry

Abstract

AbstractMass propagation of orchids is very important to do extensively due to their slower reproduction and growth naturally. Besides this, many growers do hunting expansionally on orchids especially orchid species which is having uniqueness and exclusivity characters. Moreover, orchids such as Dendrobium and Vanda also have phytochemistry such as sesquiterpenoid, alkaloid, bibenzyl, and phenolic that could be used as medicinal properties. Mass propagation could be conducted through biotechnology approaches using either conventional in vitro technique with adding plant growth regulator or orchid breeding using genetic engineering revealed by Agrobacterium-mediated transformation techniques. All of these methods are proposed in high embryogenesis of orchids. The combination of 2,4-D and ascorbic acid was absolutely required in the Agrobacterium-mediated transformation. Insertion of KNAT1 gene produced more buds in micropropagation compared to the wild-type plants. The efficiency of transformation was increased caused by acetosyringone application.KeywordsMass propagationOrchid breedingOrchid biotechnology Dendrobium Vanda