Abstract
Six endophytic fungal species belonging to genera, Aspergillus, Alternaria, Penicillium, and Fusarium, as identified morphologically, were isolated from healthy leaves, stems, and roots of the medical plant Euphorbia peplis collected from Damietta governorate of Egypt. One Fungal strain morphologically identified as A. oryzae, was selected as the most potent producer of Kojic acid, that produced in this study by agricultural waste fermentation using sugarcane bagasse, corn cobs, and rice straw. The bagasse agricultural waste fermentation using endophytic Aspergillus oryzae strain was optimized as a source of Kojic acid in a cheap, fast, and environmentally beneficial way at agitation state rather than static and pH 6.0 after 20 days of incubation at 28 °C. Ethyl acetate extract of filtrate of Aspergillus oryzae showed promising antioxidant activity against DPPH free radical, recording IC50 = 5.7. Also, the E A extract was separated using Gas Chromatography technique recording RT: 31.95 min. and MW: 142.The study aims to the fermentation biotechnology used to reduce the cost of industrial KA production through optimization of environmental conditions of the biosynthesized KA with low toxicity and high antioxidant efficiency
Highlights
Endophytes microorganisms in internal tissues of most plant species are a proven source of novel organic natural molecules, maximizing the borders of drug discovery
This study aims to reduce the cost of industrial fermentation for Kojic acid (KA) production and increment through the optimization of environmental conditions and using different cheapest agriculture raw materials
Six fungal isolates were isolated from Euphorbia peplis medicinal plant and purified until having a pure culture
Summary
Endophytes microorganisms in internal tissues of most plant species are a proven source of novel organic natural molecules, maximizing the borders of drug discovery. To the clinically acknowledged endophyte research has yielded potential drug compounds with antimicrobial, antioxidant, antiviral, antidiabetic, anti-Alzheimer's disease and immunosuppressant, and many other diseases.These evidences raises hopes to combat incurable diseases, drug resistance and other challenges related to human health. Plant and fungi are recognized as asource of natural products. Medicinal plant harbour endophytic fungi and their host produce similar photochemical. The endophytes are known to protect their host from infectious agents and provide strength to survive in harsh conditions. The potential of finding new drugs that may be effective candidates for treating newly developing diseases in humans is great (Shukla et al, 2014)
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