Biotechnological processes for continuous cultivation of microorganisms in circulation

Abstract

The article discusses a way to increase the efficiency of a wide range of anaerobic microbiological processes by introducing into the scheme of continuous cultivation of microorganisms in circulation. Recirculation allows to increase the flow rate and process productivity for the target component. At the same time, recirculation introduces additional restrictions on the initial process data - the initial substrate concentration, flow rate and volumetric recirculation rate, which in the algorithm and program must be performed in accordance with the kinetic characteristics. Based on the kinetic relationships, an algorithm and software implementation have been developed to calculate the efficiency of an anaerobic biotechnological process with substrate and biomass recirculation, provided that the product is completely separated from the recirculation stream. The calculation algorithm includes 21 variants for the practical implementation of the process, each of which can be evaluated in the above block diagram. In addition to calculating process indicators, the program contains user assistance, including a description of variables and a brief guide to working with the program. A distinctive feature of the presented approach is the flexibility of the envisaged process, which allows, without conducting an experiment, with the help of the finished software product to assess the positive effect of inclusion in the recirculation scheme.