Abstract
Lactic acid bacteria represent a worthwhile organism within the microbial consortium for the food sector, health, and biotechnological applications. They tend to offer high stability to environmental conditions, with an indicated increase in product yield, alongside their moderate antimicrobial activity. Lack of endotoxins and inclusion bodies, extracellular secretion, and surface display with other unique properties, are all winning attributes of these Gram-positive lactic acid bacteria, of which, Pediococcus is progressively becoming an attractive and promising host, as the next-generation probiotic comparable with other well-known model systems. Here, we presented the biotechnological developments in Pediococcal bacteriocin expression system, contemporary variegated models of Pediococcus and lactic acid bacteria strains as microbial cell factory, most recent applications as possible live delivery vector for use as therapeutics, as well as upsurging challenges and future perspective. With the radical introduction of artificial intelligence and neural network in Synthetic Biology, the microbial usage of lactic acid bacteria as an alternative eco-friendly strain, with safe use properties compared with the already known conventional strains is expected to see an increase in various food and biotechnological applications in years to come as it offers better hope of safety, accuracy, and higher efficiency.
Highlights
The idea that bacteria are useful can be difficult to understand
The genera Pediococcus, Streptococcus, and certain species of Lactobacillus carry out homolactic fermentation
As observed in Lactobacillus species, Pediococcus can create a number of enzymes that releases unique food flavor compounds
Summary
The idea that bacteria are useful can be difficult to understand. Today, some are known as friendly, good, or healthy bacteria that are widely used in foods, beverages, and supplements that help promote a healthy digestive tract and immune system. For introducing sgRNA sequence to target specific genetic loci To drive Cas9 expression and transcription of two nisin promoters, respectively Enhances performance in bacteria with low recombineering efficiency; Cas 9 directed toward eliminating unmodified bacterial strains (codon saturation mutagenesis and gene deletions) (100% efficiency) Replaces wild-type Cas 9 with Cas 9N10A (nickase), increasing efficiency (25%–65%) Easy insertion or deletion of genomic DNA within less time
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