Biotechnological Approach of Threatened Species Strychnos Nux-vomica L. to Standardized Nursery Techniques

Abstract

Strychnos nux-vomica belonging to family Loganiaceae. Carbohydrate, protein, oil, steroid, alkaloid, resin, strychnine and brucine were detected in phytochemical screening. It is anticipated that lipid peroxidation may provide scientific rationale for the use of S. nux-vomica as an antidiabetic plant. There is a need to develop bio-technological approach for raising nursery plants of S. nux-vomica as per International Union for Conservation of Nature and Natural Resources (IUCN) criteria. Different concentration of growth hormones to break seed dormancy, four pre treatments, different size of polythene bags, root trainer, and impact of potting mixture on the growth were adopted to determine the best treatments. The results indicated that the seed dormancy was broken when seeds were treated with 40 ppm concentration of GA3 or 100 ppm concentration of IBA hormones and soaking with warm water, the best size of polythene bag was used as medium size of polythene (25x11 cm), the best size of root trainer was used as medium cup root trainer (187 cm 3 ), and the best potting mixture was found to be farm yard manure (FYM) with soil and sand in ratio of 1:1:1. Statistical analysis was also adopted to determine of significance levels.