Abstract
The biosynthetic origin of oxygen atoms in betaenone B (1) was established by feeding experiment using [1-13C, 18O2] acetate and by cytochrome P-450 inhibitor treatment of Phoma betae; incorporation patterns and accumulation of the plausible intermediate enabled us to propose a biosynthetic pathway for (1) involving an intramolecular Diels–Alder reaction at a late stage.
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