Biosynthetic study of amphidinolide C

Abstract

The biosynthetic origins of amphidinolide C ( 1) were investigated on the basis of 13C NMR data of 13C enriched samples obtained by feeding experiments with [1- 13C], [2- 13C], and [1,2- 13C 2] sodium acetates in cultures of a dinoflagellate Amphidinium sp. These incorporation patterns suggested that amphidinolide C ( 1) was generated from four diketide chains, four acetate units, five isolated C 1 unit from C-2 of acetates, seven branched C 1 units from C-2 of acetates, and a ‘ m– m’ and a ‘ m– m– m’ units derived only from C-2 of acetates.