Abstract
Ethzanolamine plasmalogen radiolabelled mainly in the O-alkenyl moiety was prepared from cell suspension cultures of the flagellate Leishmania donovani previously incubated with [1- 14C]octadecanol over one growth period. The optimal concentration of [1- 14C]octadecanol for labelling was shown to be 1 μM, when 60% of total lipid radioactivity appeared in the 1,2-diradyl- sn-glycero-3-phosphoethanolamine fracton, with an overall yield of approx. 35%. Analysis of this fraction revealed that 93% of the label was present in O-octa-dec-1-enyl, 3% in O-alkyl and 4% in acyl moieties. A specific radioactivity of approx. 14 mCi/mmol was determined. Raising the culture medium concentration of [1- 14C]octadecanol to 2 μM yielded a product with a specific radioactivity of 25 mCi/mmol.
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