Abstract

By incubation of germinating soybeans with mevalonate-[2- 14C] (MVA), radioactivity was incorporated into four sapogenols which were identified by TLC. Unequivocal evidence for the identity of three of the four sapogenols was provided by co-crystallization to constant specific radioactivity. The partition of incorporated radioactivity into lipid- and water-soluble fractions and the pattern of radioactivity of individual sapogenols varied with the mode of administering labeled substrates to soybean seedlings, such as incubation of germinating soybeans with MVA-[2- 14C], immersion of roots into MVA-[2- 14C] or foliar application of squalene-[ 14C]. When alfalfa seedlings were incubated with MVA-[2- 14C], about two-thirds of the radioactivity incorporated into the sapogenols was associated with medicagenic acid.

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