Abstract
(−)-Kaurene-17- 14C was administered to the leaves of a Stevia rebaudiana plant, and radioactive steviol was isolated by extraction, hydrolysis, and chromatography. Its radiochemical purity was demonstrated by dilution with carrier material, recrystallization to constant specificactivity, conversion to the methyl ester, and recrystallization. The steviol methyl ester was degraded to establish that essentially all of the radioactivity was located at the 17-carbon atom.
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