BIOSYNTHESIS OF SMALL MOLECULES IN CHLOROPLASTS OF HIGHER PLANTS

Abstract

Summary1. Chloroplasts of higher plants contain enzymes which permit them to synthesize many kinds of small molecules in addition to carbohydrates.2. Either aqueous or non‐aqueous techniques may be used to isolate chloroplasts. Aqueous methods permit the isolation of chloroplasts showing high rates of photosynthesis; the organelles can be purified by means of density gradients. Non‐aqueously isolated chloroplasts cannot photosynthesize, but show good retention of low‐molecular‐weight substances and soluble enzymes.3. Whole cells photoassimilating 14CO2 show considerable formation of 14C‐labelled amino acids and lipids, but isolated chloroplasts exhibit very poor synthesis of amino acids and lipids from 14CO2.4. Chloroplasts play an important rôle in reducing nitrate to ammonia. There is controversy about the presence in chloroplasts of nitrate reductase and about the mechanism of the light‐dependent reduction of nitrate to nitrite; however, it is generally agreed that non‐cyclic electron transport directly supports reduction of nitrite to ammonia via a chloroplastic nitrite reductase.5. Chloroplasts actively assimilate inorganic nitrogen into amino acids. The assimilation reaction is either the reductive amination of α‐ketoglutarate to glutamate or the ATP‐dependent conversion of glutamate to glutamine. The enzyme glutamate synthase has recently been found to be present in chloroplasts and may play an important function in nitrogen assimilation.6. Numerous transaminases (aminotransferases) are present in chloroplasts.7. The source of α‐keto‐acid precursors of chloroplastic amino acids is unknown. It remains to be established whether chloroplasts import the required keto acids or whether some of them might be generated via an incomplete tricarboxylic‐acid cycle located in the chloroplast.8. Chloroplasts contain characteristically high levels of mono and digalactosyl diglycerides, sulpholipid and phosphatidyl glycerol. They also have large amounts of polyunsaturated fatty acids.9. Fatty acids are synthesized by the concerted action of fatty‐acid synthetase, elongases and desaturases. Two pathways have been implicated for the formation of α‐linolenic acid.10. The galactosyldiglycerides are synthesized by successive galactosylation of diglyceride. The enzymes responsible are probably located in the chloroplastic envelope.11. The other major chloroplastic acyl lipids (sulpholipid, phosphatidylglycerol and phosphatidylcholine) have not been, as yet, synthesized de novo by means of isolated chloroplast fractions. However, indirect evidence indicates that the first two are probably formed there.12. Chlorophyllide synthesis involves the formation of δ‐aminolaevulinic acid (δALA) followed by conversion of δALA to protoporphyrin IX, which is then transformed into protochlorophyll.13. Recent evidence favours the view that δALA synthesis is not mediated by δALA synthetase but by another pathway in which δALA can be derived from α‐ketoglutarate or glutamate. It has not been established whether this pathway is localized in plastids.14. Conversion of δALA to protoporphyrin IX is mediated by soluble enzymes of the plastid stroma. Membrane‐bound enzymes mediate the conversion of protoporphyrin to protochlorophyll.15. Carotenoids are synthesized from acetyl CoA via geranylgeranyl‐pyrophosphate and phytoene intermediates. Evidence has been obtained for both neurosporene and lycopene as precursors of the cyclic carotenoids.16. The overall pathway of carotenoid formation is subject to photoregulation, particularly during the development of the chloroplast.17. Carotenes are precursors of xanthophylls, the inserted oxygen being derived from molecular oxygen.18. Chloroplasts may synthesize or interconvert gibberellin hormones.