Abstract
Abstract Two routes of single-carbon (C1) biosynthesis have been described in Escherichia coli K12 previously: from serine via serine transhydroxymethylase and from glycine via a little known set of enzymes, the Glycine Cleavage Pathway. Strains deficient in both of these still have a source of C1. There therefore exists a third pathway of C1 formation. Serine appears to be the donor for the pathway. No donor of C1 other than serine of glycine appears to be of quantitative importance in E. coli K12 growing in a synthetic glucose-supplemented medium.
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