Abstract
In this study, silver nanoparticle (AgNP) synthesis was carried out using Onosma sericeum Willd. aqueous extract for the first time, with a simple, economical, and green method without the need for any other organic solvent or external reducing or stabilizing agent. A variety of AgNPs, all of different particle sizes, were synthesized by controlling the silver ion concentration, extract volume, temperature, and pH. It was determined that the optimum conditions for AgNP synthesis were 1 mM AgNO3, pH 8, 25 °C, 20 g/200 mL extract, silver nitrate, and extract ratio 5:1 (v/v). The AgNPs were defined using UV-Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The particle size distribution and zeta potential measurements of the AgNPs were measured using the dynamic light scattering (DLS) technique. It was determined that the AgNPs with a particle size of less than 10 nm showed a higher catalytic effect in the reduction of 2-nitrobenzenamine. It was also found that these nanoparticles had a cytotoxic effect on the MCF-7 breast cancer cell line depending on dosage and time. The resulting IC50 values were between 76.63 µg/mL and 169.77 µg/mL. Furthermore, the biosynthesized AgNPs showed effective antibacterial activity against the Acinetobacter baumannii bacteria. The results of the study showed that synthesized AgNPs can have a promising role in biomedical and nanobiotechnology applications.
Highlights
Metal nanoparticles are used in various fields due to their low melting point, large surface area, and different electrical and chemical properties [1]
We report a novel and green method for synthesis of AgNPs using an extract of the root of Onosma sericeum Willd. as a reducing and stabilizing agent for the controllable synthesis of particles of different sizes at room temperature
When studies in the literature were analyzed, it was found that no study had been conducted on the synthesis of AgNPs using the root of Onosma sericeum Willd.; the present study is the first of its kind
Summary
Metal nanoparticles are used in various fields due to their low melting point, large surface area, and different electrical and chemical properties [1]. Researchers continue to work extensively on silver nanoparticles (AgNPs) due to their antimicrobial and anticarcinogenic properties [7,8] In addition to these biological properties, AgNPs have attracted attention due to their catalytic and optical properties [9,10]. Green chemistry approaches have been used for synthesis of AgNPs with eco-friendly, safe, easy, and nontoxic methods For this purpose, natural resources such as plants [20], yeast [21], and bacteria [22] are used in synthesis of AgNPs. In particular, plants do not need to be combined with any other agents in obtaining metal nanoparticles, because the bioactive molecules they contain can act as capping agents [23]. We report a novel and green method for synthesis of AgNPs using an extract of the root of Onosma sericeum Willd. We chose to optimize different parameters which can affect AgNP particle size, including temperature, pH, metal salt concentration, and root extract volume. When studies in the literature were analyzed, it was found that no study had been conducted on the synthesis of AgNPs using the root of Onosma sericeum Willd.; the present study is the first of its kind
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