Biosynthesis of Silver Nanoparticles using Aspergillus Niger Isolated from Soil and Studying its Antimicrobial Effect Against Some Multidrug-Resistant Bacterial Species and Candida Albican

Abstract

Objectives: Biosynthesis of silver nanoparticles using the fungus Aspergillus niger, isolated from the soil for the purpose of studying its ability to produce nanoparticles. Methods: Grown in a liquid fermentation medium in glass flasks for ten days. The biosynthesis process was detected by observing the color changes of the filtrate. The properties of silver nanoparticles were determined by UV-measurement. Use SEM to discover the size, shape and distribution of nanoparticles. The chemical groups responsible for the biosynthesis and encapsulation of nanoparticles were detected using FTIR for both the fungal biomass filtrate and the resulting particles, after turning them into powder. Silver nanoparticles produced from mushrooms using the Well-Diffusion-Method showed antibacterial activity against MDR-bacteria for both Gram-positive and Gram-negative Bacteria, which are: (E. coli, K. Pneumonia, S. aureus, and P. aeruginosa), at two concentrations (50, 100). mg/ml) for AgNPs. Results: The biosynthesis was detected by observing the color changes of the filtrate from transparent to light yellow to dark brown. The properties of silver nanoparticles were determined by measurement (UV). Which showed absorbance values at wavelengths (450 nm). The results showed SEM The shape was spherical with dimensions ranging from (17.84-64.03) nanometers. Conclusions: The fungus Aspergillus niger showed the ability to biosynthesise silver nanoparticles after 24 hours of incubation of the fungal filtrate treated with AgNO3.