Biosynthesis of silver nanoparticles using aqueous leaf extract of Thevetia peruviana Juss and its antimicrobial activities

Omolara O Oluwaniyi,Ayomide H Labulo,Haleemat I Adegoke,Elijah T Adesuji,Charles O Oseghale,Aderemi B Alabi,Sunday O Bodede

doi:10.1007/s13204-015-0505-8

Abstract

Biosynthesizing of silver nanoparticles using microorganisms or various plant parts have proven more environmental friendly, cost-effective, energy saving and reproducible when compared to chemical and physical methods. This investigation demonstrated the plant-mediated synthesis of silver nanoparticles using the aqueous leaf extract of Thevetia peruviana. UV–Visible spectrophotometer was used to measure the surface plasmon resonance of the nanoparticles at 460 nm. Fourier Transform Infrared showed that the glycosidic –OH and carbonyl functional group present in extract were responsible for the reduction and stabilization of the silver nanoparticles. X ray diffraction, Scanning Electron Microscopy, Transmission Electron Microscopy and Selected Area Electron Diffraction analyses were used to confirm the nature, morphology and shape of the nanoparticles. The silver nanoparticles are spherical in shape with average size of 18.1 nm. The synthesized silver nanoparticles showed activity against fungal pathogens and bacteria. The zone of inhibition observed in the antimicrobial study ranged between 10 and 20 mm.

Highlights

Nanoparticles have been the subject of substantial research in recent years
Biosynthesizing of silver nanoparticles using microorganisms or various plant parts have proven more environmental friendly, cost-effective, energy saving and reproducible when compared to chemical and physical methods. This investigation demonstrated the plant-mediated synthesis of silver nanoparticles using the aqueous leaf extract of Thevetia peruviana
We explored the effectiveness of T. peruviana aqueous leaf extract as a viable source of reducing and stabilizing agent for silver nanoparticles

Summary

Introduction

Nanoparticles have been the subject of substantial research in recent years. It has been explored by researchers in various fields of science and technology (Kholoud et al 2010). This investigation demonstrated the plant-mediated synthesis of silver nanoparticles using the aqueous leaf extract of Thevetia peruviana. We explored the effectiveness of T. peruviana aqueous leaf extract as a viable source of reducing and stabilizing agent for silver nanoparticles. Synthesis of silver nanoparticles In a typical reaction procedure, 5 mL of T. peruviana aqueous leaf extract was added to 20 mL of 1 mM silver nitrate solution.

Results

Conclusion