Biosynthesis of Silver Nanoparticles through Biomass of Fungus Aspergillusniger and their Antibacterial Potential

Abstract

Extracellular biosynthesis of silver nanoparticles by fungus Aspergillus niger which was isolated from waste water is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-Vis spectrum, showing the absorbance between 260 to 400 nm. The nanoparticles characterized by Scanning Electron Microscopy exhibited silver nanoparticles with diameter of 25nm to 75nm. Energy Dispersive X-ray analysis reveals strong signals in the silver region and confirms the formation of the silver nanoparticles. The Fourier Transform Infrared Spectroscopy study confirmed that the A. niger mycomass has the ability to perform both reduction and capping functions on the silver nanoparticles. Compound Microscopy confirms the self-assembling property of silver nanoparticles. The silver nanoparticles showed remarkable antibacterial activity against Lactobacillus plantarum, Lactobacillus delbrueckii and Bacillus subtilis bacterial strains. Reduction of silver ions is an extracellular and rapid process; this information may lead to the development of easy protocols for biosynthesis of the silver nanoparticles. Antibacterial activity of silver nanoparticles is important for the development of effective antibacterial agent against those bacteria who are showing resistance against antibiotic drugs which are available in market.