Abstract
Silver nanoparticles play an integral part in the evolution of new antimicrobials against the broad ranges of pathogenic microorganisms. Recently, biological synthesis of metal nanoparticles using plant extracts has been successfully consummated. In the present study, the biosynthesis of silver nanoparticles (AgNPs) was conducted using the leaf extract of plant Protium serratum, having novel ethnomedicinal. The synthesized AgNPs were characterized using UV-Visible spectroscopy, dynamic light scattering spectroscopy (DLS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy. The DLS study revealed the surface charge of the resulted nanoparticles that was highly negative, i.e., −25.0 ± 7.84 mV and the size was 74.56 ± 0.46 nm. The phytochemical and FTIR analysis confirmed the role of water-soluble phyto-compounds for the reduction of silver ions to silver nanoparticles. The potential antibacterial activity of AgNPs was studied against the food borne pathogens viz. Pseudomonas aeruginosa (IC50 = 74.26 ± 0.14 μg/ml), Escherichia coli (IC50 = 84.28 ± 0.36 μg/ml), Bacillus subtilis (IC50 = 94.43 ± 0.4236 μg/ml). The in vitro antioxidant potential of AgNPs was evaluated using 1, 1-diphenyl-2-picryl-hydrazil (IC50 = 6.78 ± 0.15 μg/ml) and hydroxyl radical assay (IC50 = 89.58 ± 1.15 μg/ml). In addition, the cytotoxicity of AgNPs was performed against fibroblast cell line L-929 to evaluate their biocompatibility. The overall results of the present investigation displayed the potential use of P. serratum leaf extract as a good bio-resource for the biosynthesis of AgNPs and their implementation in diverse applications, specifically as antibacterial agent in food packaging and preservation to combat against various food borne pathogenic bacteria along with its pharmaceutical and biomedical applications.
Highlights
The phyto-mediated synthesis is a rapid, suitable and most acceptable biosynthetic method for synthesis of metal nanoparticles
The present study reported the biological synthesis of AgNPs using the cell-free aqueous leaf extract of P. serratum and evaluation of its potential application as an antibacterial agent against two Gram negative (Pseudomonas aeruginosa MTCC 2453 and Escherichia coli MTCC 739) and two Gram positive (Bacillus subtilis MTCC 736, Staphylococcus aureus MTCC 2940) food borne pathogenic bacteria along with antioxidant potentials in terms of DPPH and OH radical scavenging activity
The UV–vis spectroscopy is an indirect method to examine the bioreduction of Ag nanoparticles from aqueous AgNO3
Summary
The phyto-mediated synthesis is a rapid, suitable and most acceptable biosynthetic method for synthesis of metal nanoparticles. There are several reports on synthesis of green silver nanoparticles (AgNPs) using various plant products.
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