Abstract
New applications of nanomaterials are rapidly emerging. The synthesis of nanoparticles is a cornerstone of nanotechnology. Microbial cells are highlyorganized units, regarding morphology and metabolic pathways, capable of synthesizing well size-calibrated and well-structured particles. Furthermore, biogenic nanoparticles often are water-soluble and biocompatible, which is essential for many applications. Molecular identification of a novel strain P. fluorescens BCPBMS-1 from sponge Callysspongia diffusa (Mandapam Coast) through 16s rRNA ribotyping (Gen bank accession number: HQ907732). The silver nanoparticles were analyzed by UV-Visible spectroscopy. Their chemical composition was determined by FT-IR spectroscopy. SEM observationrevealed that silver nano particles are having spherical shape. The antibacterial activities of silver nanoparticles were screened against common human pathogen Escherichia coli, Proteus mirabilis, Salmonella typhi, Salmonella paratyphi, Vibrio cholerae, Klebsiella oxytoca, Klebsiella pneumoniae and Staphylococcus aureus. Among these 5mm antibacterial activity was observed with E. coli, 4mm with P. mirabilis and S. typhi, 3mm activity was observed with S. paratyphi. These results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.
Highlights
Marine microbial biotechnology has opened up unexpected new horizons for finding novel organism for trapping their potential resources .Oceans account for more than 70% of the earth’s surface and the microorganisms growing in marine environments are metabolically and physiologically diverse from the terrestrial organisms (Takizawa et al, 1993)
Ag-NPs are used in hygienic products including water Purification systems, linings of washing machine, dishwashers, refrigerators, and toilet seats (Rai et al, 2009) silver ions have been used in many kinds of formulations (Sondi & Salopek-Sondi, 2004), and recently it was shown that hybrids of silver nanoparticles with amphiphilic hyper branched macromolecules exhibit effective antimicrobial surface coating (Ymonier et al, 2002)
In the present study Phylogenetic tree revealed that P. fluorescens BCPBMS-1 which was isolated from marine sponge Callyspongia diffusa was closely related to P. fluorescens CIAH-Pf-196-16s strain (Fig. 1)
Summary
Marine microbial biotechnology has opened up unexpected new horizons for finding novel organism for trapping their potential resources .Oceans account for more than 70% of the earth’s surface and the microorganisms growing in marine environments are metabolically and physiologically diverse from the terrestrial organisms (Takizawa et al, 1993).Ag-NPs (silver nanoparticles) are used in hygienic products including water Purification systems, linings of washing machine, dishwashers, refrigerators, and toilet seats (Rai et al, 2009) silver ions have been used in many kinds of formulations (Sondi & Salopek-Sondi, 2004), and recently it was shown that hybrids of silver nanoparticles with amphiphilic hyper branched macromolecules exhibit effective antimicrobial surface coating (Ymonier et al, 2002). Marine microbial biotechnology has opened up unexpected new horizons for finding novel organism for trapping their potential resources .Oceans account for more than 70% of the earth’s surface and the microorganisms growing in marine environments are metabolically and physiologically diverse from the terrestrial organisms (Takizawa et al, 1993). One of the most studied aspects of nanotechnology nowadays is their ability to offer the opportunity to fight microbial infections via synthesis of nanoparticles. The mechanism of prevention of bacterial growth by antibiotics is quite different from the mechanisms by which nanoparticles inhibit microbial growth. Nanoparticles have the potential to serve as an alternative to antibiotics and to control microbial infections. Biosynthesis of silver Nanoparticles from marine sponge C. diffusa associated - P. fluorescens 41
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