Abstract
Development of natural based nanomaterial immense interest of researchers involved in eco-friendly biosynthesis of nanoparticles. This research set out to investigate the potential of Allium ampeloprasum leaves extract to synthesize silver nanoparticles (AgNPs), and evaluate their antifungal activity against some toxigenic strains of Aspergillus isolates. In order to achieve this, AgNPs were prepared using plant extracts at room temperature and the formation of AgNPs was visually detected after the color changed to dark brown within few minutes. The biosynthesized AgNPs were characterized using the UV-vis spectroscopy, which confirmed the formation of AgNPs with a maximum peak at 437 nm due to the Plasmon resonance. The main active reduction agents were detected by Fourier Transmission infrared spectroscopy (FTIR). Also, the nanoparticles were characterized using Scanning electron microscope (SEM). The antifungal activity of AgNPs was investigated by agar well diffusion method, which revealed inhibition zones of 8 mm, 10 mm, 11 mm, 11 mm and 14 mm against Aspergillus flavus 1, A. parasiticus, A. flavus 2, A. ochraceus, and A. niger. The minimum inhibition concentration (MIC) was determined using micro broth dilution method. MIC values of AgNPs ranged from 652 - 2500 μg/ml. The fungi treated with AgNPs, were examined with (SEM), it was observed that the treated fungi were damaged. These results suggest that AgNPs have the potential to be used as an ideal eco-friendly approach to control toxigenic fungi.
Highlights
Toxigenic fungi pose significant health hazards to humans and animals worldwide
This research set out to investigate the potential of Allium ampeloprasum leaves extract to synthesize silver nanoparticles (AgNPs), and evaluate their antifungal activity against some toxigenic strains of Aspergillus isolates
These results suggest that AgNPs have the potential to be used as an ideal eco-friendly approach to control toxigenic fungi
Summary
Toxigenic fungi pose significant health hazards to humans and animals worldwide. These toxigenic fungi contaminate food products and produce mycotoxins as secondary metabolites, such as Aspergillus species which is considered as the most significant producers of mycotoxins [1] [2] [3] [4].Mycotoxins are considered as an important reason for the illness of human as well as farm animals around the world. Toxigenic fungi pose significant health hazards to humans and animals worldwide. These toxigenic fungi contaminate food products and produce mycotoxins as secondary metabolites, such as Aspergillus species which is considered as the most significant producers of mycotoxins [1] [2] [3] [4]. Mycotoxins are considered as an important reason for the illness of human as well as farm animals around the world. The issue of fungal resistance and health hazard due to exposure to chemical fungicides is a major concern. Studies have been carried out to circumvent this issue by developing new technologies to control the contaminant fungi such as using metal nanoparticles as antimicrobial agents [6] [7] [8]
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