Abstract
Changes in the biosynthesis of serine proteases in adult Lutzomyia anthophora Addis were followed and compared with the larval and pupal stages. More chymotrypsinlike than trypsinlike enzyme was synthesized by 2-d-old and 3-d-old sugar-fed females and females that were fed blood 72 h earlier. A small increase in the amount of chymotrypsinlike enzyme occurred within the first 48 h after blood feeding, whereas trypsinlike enzyme activity increased rapidly after the blood meal and peaked at 72 h. [1,3-3H]DIP trypsinlike and chymotrypsinlike derivatives of sugar-fed and blood-fed females were compared using polyacrylamide gel electrophoresis.
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