Abstract
Salvia divinorum plants were exposed to a synthetic atmosphere containing 600 ppm 13CO2 for 4 hours with white light illumination and were subsequently cultured for a chase period of 10 days under standard greenhouse conditions. Salvinorin A was obtained by solvent extraction of the leaves and was purified chromatographically. The isotopolgue composition of salvinorin A was determined by NMR spectroscopy. The presence of multiply 13C-labeled isotopologues in considerable excess over their natural occurrence showed that the 13C labeling pulse had afforded multiply 13C-labeled biosynthate. Consecutive catabolic and anabolic reactions during the chase period afforded salvinorin A that was a mosaic revealing its origin from a mixture of labeled and unlabeled precursors. The presence of triple labeled isotopologues demonstrated that the IPP and DMAPP precursors of the diterpene, salvinorin A, were predominantly biosynthesized via the recently discovered non-mevalonate pathway.
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Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
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