Abstract
One strain of Pseudomonas andropogonis incorporated radioactivity from l-[U- 14C]-aspartic acid into l- threo-hydroxythreonine, and three strains incorporated radioactivity into both l- threo-hydroxythreonine and rhizobitoxine. l-[ 14C]- threo-hydroxythreonine was isolated from a culture of P. andropogonis that had been provided with l-[U- 14C]-aspartic acid for 35 min, and purified. When this was fed to a rhizobitoxine-producing strain of P. andropogonis, < 20% of the radioactivity supplied was removed from the medium in 5–6 hr. Radioactivity in the medium was found to be in hydroxythreonine and rhizobitoxine. A high level of the utilized radioactivity from hydroxythreonine had been incorporated into rhizobitoxine. These data support the proposal that biosynthesis of rhizobitoxine proceeds through l-aspartic acid and that hydroxythreonine is an intermediate en route to rhizo-bitoxine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.