Abstract
The effect of methylation on the translation of reovirus messenger RNA catalyzed by the homologous mouse ascites tumor and the heterologous wheat embryo cell-free protein synthesizing systems was examined. Unmethylated and methylated reovirus mRNAs were translated with comparable efficiency in the ascites system; by contrast, in the wheat system, unmethylated reovirus mRNA was translated about 10% as efficiently as methylated viral mRNA. S-Adenosyl- l-homocysteine (SAdoHcy) inhibited S-adenosyl- l-[ Me- 3H]methionine-mediated methylation in both the ascites and wheat cell-free extracts; however, translation of unmethylated reovirus mRNA was blocked by SAdoHcy in the wheat, but not in the ascites, cell-free protein synthesizing system.
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