Abstract
Polyhydroxyalkanoates (PHAs) are the most promising alternatives to petroleum-derived polymers. The present study focuses on PHA synthesis by a new wild-type bacterial strain using lactose as a carbon source. Staining microbial cells with Sudan Black B (SBB) and Nile Blue A (NBA) yielded positive PHA granules. Bacillus tequilensis ARY86 was identified using 16S rRNA gene sequence analysis and deposited in the NCBI GenBank database. ARY86 showed a maximum yield of PHA 62% (w/w) by using 2% lactose and 0.5% nitrogen (C/N ratio of 8:1) at 30 °C and pH 7 as optimal conditions. The extracted polymer was characterized using FTIR and GC–MS to observe the functional groups in PHA. The DSC and XRD analysis to investigate thermal properties and crystalline nature of PHA respectively. The melting point was found to be 174.5 °C while the degree of crystallinity was 43.7%–45.7%, indicating the Scl-co-mcl PHA structure that offers a wide range of applications.
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