Abstract
Phosphate labelled with P32 was fed to viable wheat embryos with and without 5-amino-4-imidazole carboxamide (AICA). The cold perchloric acid fraction was treated with Norit A; the adsorbed nucleotides were eluted by pyridine–alcohol and subjected to chromatographic analysis for identification. Approximately 30% of the label in the soluble fraction was incorporated into identifiable purine and pyrimidine nucleotides. AICA had no effect on the amount of incorporation. This study shows the existence of a pool of free nucleotides in wheat embryos.
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