Abstract
Summary The overall biosynthesis of methionine has been examined in different growth conditions and different strains, including regulatory mutants. The following points can be stressed: (1) The pool size of free methionine remains remarkably constant during the exponential phase of growth in wild type strains as well as in methionine overproducers (see below). 2) Methionine accumulates in the pool of some regulatory mutants, the most extreme one, bearing the mutation eth2-2, having a 20 fold higher methionine pool size than its corresponding wild type. 3) Accumulation is accounted for by increased rates of synthesis observed in these regulatory mutants. 4) A part of the methionine overproduced is excreted in the medium. A part of it is probably metabolized further. 5) Under conditions of either methionine-mediated repression or S-adenosylmethioninemediated repression, kinetics of decrease of methionine biosynthesized are compatible with repression being the major regulatory mechanism for this biosynthetic pathway in Sacch. cerevisiae.
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