Abstract
Cultures of Fusarium oxysporum Schl. emend. Sny. et Hans. f lycopersici (Sacc.) Sny. et Hans. grown on a liquid medium with glucose as the principal carbon source produce, among other products, the phytotoxin lycomarasmin. Acid hydrolysis of lycomarasmin results in the formation of aspartic acid, glycine, and pyruvic acid. Tracer studies showed that glycine-U-I4C, L-serine-U-14C, DL-aspartic acid-4-14C, DL-alanine-1-14C, and glucose-U-14C served as relatively efficient precursors of the lycomarasmin molecule. DL-Aspartic acid-4-I4C was incorporated into the 4-carbon fragment without label scrambling. Glycine was found to be the most efficient precursor of the 2-carbon fragment while glucose afforded the most efficient and direct route for the 3-carbon fragment.
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