Abstract

Enzymatic elongation of behenyl-CoA has been studied in mouse brain microsomes. Malonyl-CoA and reduced nicotine-adenine-dinucleotide phosphate are required. The effect of protein concentration and incubation time have been determined. Phosphotransacetylase was added to reduce the de novo microsomal system and the radioactive fatty acids synthesized were identified by thin layer chromatography and gas chromatography with automatic counting of the eluate. The elongation capacity of Quaking microsomes is reduced to 30% of the normal value.

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