Abstract
The potential of palm kernel cake (PKC) wastes as feedstocks for the production of hyaluronic acid (HA) using engineered Escherichia coli has been investigated. Herein, we report a novel thermophilic exo-β-D-mannanase TFMS, which exhibits superior enzymatic properties and more efficiently hydrolyzes PKC to yield oligosaccharides composed of 1-m to 10-m monosaccharide residues. By optimizing signal peptides, we achieved high-level secretory production of TFMS in engineered chassis strain, which could effectively release oligosaccharides from PKC for cell growth. Our results confirm that the chassis strain maintains relatively high cell-growth rates and biomass in PKC media with approximately 70.5% levels of that in LB media. Furthermore, hasA, a HA synthase gene from Streptococcus zooepidemicus, was expressed in chassis strain to produce a relatively high HA accumulation of 192 mg/L from PKC. This study provides a new approach for developing feedstock utilizations of PKC wastes by constructing efficient microbial cell factories to biosynthesize value-added products.
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