Abstract
Cytokinins were detected in culture nitrates of Azotobacter chroococcum, A. beijerinckii, A. vinelandii, Pseudomonas fluorescens and P. putida. The most prolific producer of cytokinins was A. chroococcum. Several purine ring constituents [adenine (ADE), adenosine, adenosine-5′-monophosphate, hypoxanthine, inosine and inosine-5′-monophosphate] and isoprenoid compounds [isopentyl alcohol (IA), 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol and isoprene] were tested for their suitability as physiological precursors to cytokinin biosynthesis. The isolation and quantification of zeatin riboside, dihydrozeatin riboside, t- and c-zeatin, isopentyl adenine and its riboside were monitored by high performance liquid chromatography (HPLC), u.v. spectrometry and bioassay. The stimulation of A. chroococcum to produce cytokinins by the addition of ADE and IA was monitored during the growth of the bacterium and tested under various environmental conditions in liquid media.
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