Abstract
Comparative feeding experiments have been performed to investigate the biosynthesis of coumestrol in mung bean seedlings ( Phaseolus aurues Roxb.). 4′,7-Dihydroxyisoflavone-[4- 14C] (daidzein) and 2′,4,4′- trihydroxychalcone-[β- 14C] were good precursors for coumestrol, whereas o-coumaric acid-[3- 14C] was incorporated to a small extent and 3-(4-hydroxyphenyl)-4,7-dihydroxycoumarin-[4- 14C] was not incorporated at all. Although 2,2′,4,4′-tetrahydroxychalcone-[6T,βT] was incorporated, this compound does not appear to be a natural intermediate. Dilution analyses with 2′,4′,7-trihydroxyisoflavone suggests that 2′-hydroxylation of daidzein or dihydrodaidzein is a probable step in the biosynthesis of coumestrol.
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