Abstract
Copper nanoclusters (Cu NCs) have shown significant attention in sensing of molecular and ionic species. In this work, a single-step biosynthetic approach was introduced for the preparation of fluorescent Cu NCs using Holarrhena pubescens (H.pubescens) leaves extract as a template. The synthesized H.pubescens-Cu NCs act as a nanomolecular probe for the detection of bilirubin in biofluids. The synthesized H.pubescens-Cu NCs displayed highest fluorescence intensity at 454 nm, when excited at 330 nm. Importantly, selective detection of bilirubin was obtained by introducing H.pubescens-Cu NCs as a simple molecular probe. The interaction of bilirubin and H.pubescens-Cu NCs resulted in a remarkable decrease in the emission peak intensity. The developed H.pubescens-Cu NCs-based bilirubin molecular probe has a wide linear range of 0.5-20.00 μM with the limit of detection of 30.54 nM for bilirubin. The promising application of H.pubescens-Cu NCs-based molecular probe was assessed by assaying bilirubin in spiked biofluids.
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