Abstract

Phyto fabricated nanoparticles, bearing the advantage of possessing the plants’ medicinal values and totally following the green approach in nanomaterial synthesis is gaining popularity in the scientific world. The present work investigates the green synthesis of zinc oxide nanoparticles using chitosan and Eupatorium adenophorum. Different spectroscopic and imaging techniques such as UV–Vis, FTIR, XRD, FE-SEM, EDAX, HRTEM and SAED were used to evaluate the structural morphology of the zinc oxide nanoparticles. The UV–Vis spectroscopy displayed absorbance peak at 355 nm. TEM and SEM images showed spherical and hexagonal morphology with average size of 42.60 nm. The synthesized nanoparticles were tested for antibacterial activity and the results showed effective antibacterial potential with a maximum zone of inhibition of 25 mm against Staphylococcus aureus. The maximum DPPH radical scavenging activity was 91.4% at the highest nanoparticle concentration while the α-amylase assay for antidiabetic test showed 68% inhibition at 25 μg/mL concentration of the nanoparticles. After 10 h, the percentage of congo red dye being photocatalytically degraded was found to be 93.4%. To the best of our knowledge this is the first ever study on Chitosan and Eupatorium adenophorum phytochemical mediated green synthesis of ZnO nanoparticles and evaluation of their biological and photocatalytic efficacy.

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