Abstract
Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty.
Highlights
The chemical purity and unique architecture of bacterial cellulose (BC) provide BC with properties such as high degree of polymerization, high crystallinity, mechanical strength, elasticity and shapeability, gas and liquid permeability, hydrophilicity, high water-holding capacity, nanoscale dimension, and nanoporosity [1,2]
The present study aimed to explore the biosynthesis of BC by an extended cultivation method with multiple removal of BC pellicles where glucose is added once
Besides the high surface areas of BC gel-films obtained by pellicle removals II and III, the degree of polymerization and elastic modulus increased as compared to the single-time removal of a BC pellicle
Summary
The chemical purity and unique architecture of bacterial cellulose (BC) provide BC with properties such as high degree of polymerization, high crystallinity, mechanical strength, elasticity and shapeability, gas and liquid permeability, hydrophilicity, high water-holding capacity, nanoscale dimension, and nanoporosity [1,2]. We advanced a hypothesis that thin BC gel-films could repeatedly be taken out of the surface of the culture medium having an initial substrate loading that is optimum for BC biosynthesis (it is usually 20 g/L) [1]. BC synthesis is known to be stimulated when the cell concentration rises and the cells reach a quorum [16,34] Such gel-films with a greater surface area are demanded in prosthetic hernia repair of the anterior abdominal wall. The resultant gel-films were tested in prosthetic tension-free hernioplasty
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