Abstract

( R,S)-[1- 14C, 15N]Methionine, ( R,S)-[1- 14C, 2- 15N]- and ( R,S)-[1- 14C, 4- 15N]-2,4-diaminobutanoic acid, and [1- 13C, carboxyl- 14C, 15N]-1-aminocyclopropane-1-carboxylic acid were synthesized and fed to Convallaria majalis plants. The labelled methionine was the most efficient precursor of azetidine-2-carboxylic acid (A-2-C), the specific incorporation of the 15N (0.47%) being essentially the same as that of the 14C (0.45%). The A-2-C derived from the labelled 2,4-diaminobutanoic acid was radioactive (specific incorporation of 14C: 0.05, 0.06%); however, a much higher level of activity was found in aspartic and glutamic acid, and 2,4-diaminobutanoic acid is not considered to be a direct precursor of A-2-C in this species. Negligible 14C activity was found in A-2-C after feeding the labelled 1-aminocyclopropane 1-carboxylic acid.

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