Abstract
The high level incorporation of isotopically labelled sodium acetate and sodium propionate into amphotericin B is reported. Double quantum filtered heteronuclear single quantum coherence NMR (DQF-HSQC) is employed to demonstrate coupling between labelled carbons on intact acetate units after biosynthesis. Electrospray mass spectroscopy demonstrates the high level incorporation of label is present in only half the molecules, the other half remaining unlabelled.
Highlights
1.1 Background1.2 Evolution o f polyketides1.2.2 Fatty acid biosynthesis1.3 Polyketide biosynthesis1.3.1 Aromatic polyketides1.3.2 Non-aromatic polyketides1.3.2.1 Processive v& non-processive hypothesis1.3.2.2 Evidence for processive hypothesis1.3.2.3 Further evidence: genetic analysis1.4 A m photericin B
The biosynthesis o f amphotericin B in the present study was investigated by a number o f methods that have previously been used for the elucidation o f biosynthetic pathways for various polyketide metabolites
Tim e of Inoculation/h sources are utilised during different stages o f the fermentation process, with the fructose being used during the growth period and collofilm dextrin during the production period^ It might be considered that the complex media produces less due to the faet that the carbon source is limiting, but when more glucose is added in the m edia formulation, the concentration of amphotericin B does not increase measurably
Summary
Ephedrine 3; respiratory ailments Ephedra spp. Ricinine 4 (castor oil): purgative Rîcinus communis L. Q uinine 5: Antimalarial Cinchona officinalis L. Many secondary metabolites are molecules that are both structurally and pharm acologically interesting. W ithin the sub-group o f polyketides alone there is great diversity in the range o f chemical structures and biological activity (Fig. 1.2). These include antibiotics {e.g. erythromycin A 6 and tetracycline 7), antifungal agents (griseofulvin 8 and amphotericin B 9), anti-cancer agents (dynemycin 10) and im munosuppressives (rapamycin 11). Me.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
