Abstract
Incubation of UDP-[ 14C]- N -acetylglucosamine with calf pancreas microsomes in the presence of Mn ++ and potassium thiocyanate gave a labeled glycolipid, tentatively identified as P 1-2-acetamido-2-deoxy- D -glucosyl P 2-dolichyl pyrophosphate on the basis of cochromatography with synthetic P 1-2-acetamido-2-deoxy-α- D -glucopyranosyl P 2-dolichyl pyrophosphate, similar chemical and enzymic hydrolyses of the biosynthetic and synthetic compounds, and stimulation of the biosynthesis by addition to the incubation mixture o dolichyl phosphate or a crude lipid fraction extracted from microsomes.
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