Abstract

Feeding of [ 13C 2]acetate to hairy roots of Ajuga reptans var. atropurpurea followed by 13C-NMR analysis of the biosynthesized cholesterol and 20-hydroxyecdysone indicated that C-25 hydroxylation in 20-hydroxyecdysone biosynthesis proceeds both in retention and inversion mechanisms. Feeding studies of [26- 13C]- and [27- 13C]cholesterols established that the ratio of retention and inversion mechanisms is ca. 3:1.

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